Journal: International Journal of Clinical and Experimental Pathology

Article Title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression

doi:

Figure Lengend Snippet: Representative immunohistochemical expression for NOD1, RIP2, Caspase12, hBD1, hBD2 and hBD3. NOD1 is stained in cytoplasm and nucleus shown with strong staining in the whole epithelium of normal oral specimens (A), moderate cytoplasm staining mainly in the granular layers and cuticular layer of OLK tissues (B), and weak staining in OSCC (C). RIP2 is stained in cytoplasm shown with moderate staining in normal oral epithelium (D), weak staining in OLK (E), and no staining in OSCC (F). Caspase12 (G-I), hBD1 (J-L) and hBD2 (M-O) are shown with cytoplasmic and nuclear staining, while hBD3 is mainly shown with cytoplasmic staining (P-R). Representative Caspase12 expression is shown with extremely strong staining intensity in the basal layer and strong staining intensity in the spinous layer of normal oral epithelium (G), strong staining intensity in OLK (H) and weak staining intensity in OSCC (I). hBD1 is detected in the squamous epithelium shown with strong staining in normal oral specimens (J), strong staining in OLK (K), and absent staining in OSCC (L). hBD2 is detected in the squamous epithelium shown with strong staining in normal oral specimens (M), strong staining in OLK (N), and weak staining in OSCC (O). hBD3 is detected in the squamous epithelium shown with strong staining in normal oral specimens (P), strong staining in OLK (Q), and absent staining in OSCC (R). Original magnification: 100×.

Article Snippet: Primary antibodies used were rabbit polyclonal anti-NOD1 (1:200; Abcam), mouse monoclonal anti-RIP2 (1:50 dilution; Abcam), rabbit polyclonal anti-Caspase12 (1:800 dilution; Abcam), mouse monoclonal anti-hBD1 (1:300 dilution; Abcam), rabbit polyclonal anti-hBD2 (1:500 dilution; Abcam), and rabbit polyclonal anti-hBD3 (1:400 dilution; Novus).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression

doi:

Figure Lengend Snippet: Association of immunostaining scores of NOD1, RIP2, Caspase12 and hBD1, 2, 3 and OSCC progression

Article Snippet: Primary antibodies used were rabbit polyclonal anti-NOD1 (1:200; Abcam), mouse monoclonal anti-RIP2 (1:50 dilution; Abcam), rabbit polyclonal anti-Caspase12 (1:800 dilution; Abcam), mouse monoclonal anti-hBD1 (1:300 dilution; Abcam), rabbit polyclonal anti-hBD2 (1:500 dilution; Abcam), and rabbit polyclonal anti-hBD3 (1:400 dilution; Novus).

Techniques: Immunostaining, Diagnostic Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression

doi:

Figure Lengend Snippet: Association of immunostaining scores of NOD1, RIP2, Caspase12 and hBD1, 2, 3 and tumor differentiation of OSCC

Article Snippet: Primary antibodies used were rabbit polyclonal anti-NOD1 (1:200; Abcam), mouse monoclonal anti-RIP2 (1:50 dilution; Abcam), rabbit polyclonal anti-Caspase12 (1:800 dilution; Abcam), mouse monoclonal anti-hBD1 (1:300 dilution; Abcam), rabbit polyclonal anti-hBD2 (1:500 dilution; Abcam), and rabbit polyclonal anti-hBD3 (1:400 dilution; Novus).

Techniques: Immunostaining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression

doi:

Figure Lengend Snippet: Association of immunostaining scores of NOD1, RIP2, Caspase12 and hBD1, 2, 3 and lymph node metastasis of OSCC

Article Snippet: Primary antibodies used were rabbit polyclonal anti-NOD1 (1:200; Abcam), mouse monoclonal anti-RIP2 (1:50 dilution; Abcam), rabbit polyclonal anti-Caspase12 (1:800 dilution; Abcam), mouse monoclonal anti-hBD1 (1:300 dilution; Abcam), rabbit polyclonal anti-hBD2 (1:500 dilution; Abcam), and rabbit polyclonal anti-hBD3 (1:400 dilution; Novus).

Techniques: Immunostaining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression

doi:

Figure Lengend Snippet: Association of immunostaining scores of NOD1, RIP2, Caspase12 and hBD1, 2, 3 and tumor size of OSCC

Article Snippet: Primary antibodies used were rabbit polyclonal anti-NOD1 (1:200; Abcam), mouse monoclonal anti-RIP2 (1:50 dilution; Abcam), rabbit polyclonal anti-Caspase12 (1:800 dilution; Abcam), mouse monoclonal anti-hBD1 (1:300 dilution; Abcam), rabbit polyclonal anti-hBD2 (1:500 dilution; Abcam), and rabbit polyclonal anti-hBD3 (1:400 dilution; Novus).

Techniques: Immunostaining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression

doi:

Figure Lengend Snippet: Association of immunostaining scores of NOD1, RIP2, Caspase12 and hBD1, 2, 3 and dysplasia grade of OLK

Article Snippet: Primary antibodies used were rabbit polyclonal anti-NOD1 (1:200; Abcam), mouse monoclonal anti-RIP2 (1:50 dilution; Abcam), rabbit polyclonal anti-Caspase12 (1:800 dilution; Abcam), mouse monoclonal anti-hBD1 (1:300 dilution; Abcam), rabbit polyclonal anti-hBD2 (1:500 dilution; Abcam), and rabbit polyclonal anti-hBD3 (1:400 dilution; Novus).

Techniques: Immunostaining

Journal: ACS Omega

Article Title: Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

doi: 10.1021/acsomega.1c01576

Figure Lengend Snippet: Proliferation of drNPCs on the SBEM with or without the ECM-peptide motifs in the growth medium. drNPCs on all the SBEM intensively expressed Nestin (A–D), whereas the percentage of SOX2-positive cells (E–H) and the co-expression of βIII-tubulin and GFAP (I–L) were significantly higher in the control group. The cells were stained with cocktails of primary antibodies (mouse monoclonal + rabbit polyclonal) followed by cocktail of goat-anti-mouse and goat-anti-rabbit secondary antibodies with Alexa Fluor 488 (green) and Alexa Fluor 633 (red), respectively. The cell nuclei were stained with Hoechst (blue) in all the panels. Laser scanning confocal microscopy. Bar size = 20–100 μm.

Article Snippet: PE-conjugated mouse anti-SOX2 (SOX2, #560291, BD Biosciences, 5 μg/mL) antibodies were also used.

Techniques: Expressing, Staining, Confocal Microscopy

Journal: ACS Omega

Article Title: Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

doi: 10.1021/acsomega.1c01576

Figure Lengend Snippet: Immunophenotyping assay of drNPCs cultured on the SBEM with or without the ECM-peptide motifs. (A) Percent of the SOX2-positive cells in spontaneous and induced differentiation of the drNPCs. (B, C) Number of cells, which expressed only GFAP + and βIII-tubulin + markers after spontaneous and induced differentiation. (D) Amount of the drNPCs, which co-expressed the neural and glial markers.

Article Snippet: PE-conjugated mouse anti-SOX2 (SOX2, #560291, BD Biosciences, 5 μg/mL) antibodies were also used.

Techniques: Cell Culture

Journal: Stem Cell Research

Article Title: IPSC reprogramming of two patients with spondyloepiphyseal dysplasia congenita (SEDC)

doi: 10.1016/j.scr.2023.103080

Figure Lengend Snippet: Reagents details.

Article Snippet: Pluripotency Markers , Mouse anti-TRA1-60 Rabbit anti-OCT4 Rabbit anti-SOX2 Mouse anti-TRA1-81 Rabbit anti-NANOG , 1:200 1:200 1:200 1:200 1:500 , Cell Signaling Technology Cat#4746S Cell Signaling Technology Cat# 2750S Abcam Cat# ab97959 Cell Signaling Technology Cat#4745S ThermoFisher Scientific Cat#PA1-097 , AB_2119059 AB_823583 AB_2341193 AB_2119060 AB_2539867.

Techniques: Immunocytochemistry, Mutagenesis, Sequencing, Virus

Journal: Stem cells (Dayton, Ohio)

Article Title: DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.

doi: 10.1002/stem.2252

Figure Lengend Snippet: Figure 1. Differences in DPPA5 gene expression and protein levels in human pluripotent stem cells (hPSCs) cultured on irradiated MEFs and feeder-free substrates. (A): Relative transcript levels of DPPA5 were higher in human embryonic stem cells (hESCs) (H9) cultured on PMEDSAH compared to that on MEFs, while relative transcript levels of OCT4, SOX2, KLF4, C-MYC, and NANOG showed no significant differences in expression. (B): Relative transcript levels of DPPA5 were higher in multiple types of hPSCs (H9, CHB10, hFF-iPSCs, hGF2- iPSCs, and hGF4-iPSCs) cultured on PMEDSAH compared to MEFs. (C): Western blot analysis and relative signal intensity of DPPA5, NANOG, OCT4, SOX2, KLF4, and C-MYC proteins in hESCs cultured on MEFs and PMEDSAH. a-Tubulin was used as a loading control. (D): Compared to when cultured on MEFs, the relative transcript levels of DPPA5 were higher in hESCs (CHB10) cultured on feeder-free sub- strates such as PMEDSAH, Matrigel, Laminin, and Vitronectin. Plot data presented as mean 6 SD from three independent experiments (*, p < .05). Abbreviations: DPPA5, developmental pluripotency associated 5; hGF, human gingival fibroblasts; hFF, human foreskin fibro- blasts; iPSCs, induced pluripotent stem cells; MEFs, mouse embryonic fibroblasts; PMEDSAH, poly[2-(methacryloyloxy) ethyl dimethyl-(3- sulfopropyl) ammonium hydroxide].

Article Snippet: Meanwhile, NANOG antibody (Cat. ab62734, Abcam, Cambridge, U.K., http://www.abcam.com), DDK antibody (Cat. TA50011, OriGene), OCT4 antibody (Cat. 4286, Cell Signaling), SOX2 antibody (Cat. 2748, Cell Signaling), normal mouse IgG (Santa Cruz Biotechnology) or normal rabbit IgG (Santa Cruz Biotechnology) were mixed and incubated to form IP antibodyIP matrix complex and the control-IP matrix complex, respectively.

Techniques: Gene Expression, Cell Culture, Irradiation, Expressing, Western Blot, Control

Journal: Stem cells (Dayton, Ohio)

Article Title: DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.

doi: 10.1002/stem.2252

Figure Lengend Snippet: Figure 3. Differences in DPPA5 expression between undifferenti- ated and differentiated human pluripotent stem cells and somatic cells. (A): Relative transcript levels of DPPA5 increased in iPSCs (hFF-iPSCs, hGF2-iPSCs, and hGF4-iPSCs) compared to correspond- ing parental fibroblasts. (B): Relative transcript levels of OCT4, SOX2, and DPPA5 decreased after EB formation from hESCs. (C): Relative transcript levels of DPPA5 decreased after directed cell lineage differentiation from hESCs into endodermal, mesodermal, and ectodermal derivatives. Data presented as mean 6 SD from three independent experiments (*, p < .05). Abbreviations: DPPA5, developmental pluripotency associated 5; EB, embryoid body; hESCs, human embryonic stem cells; hFF, human foreskin fibroblasts; hGF, human gingival fibroblasts; iPSCs, induced pluri- potent stem cells.

Article Snippet: Meanwhile, NANOG antibody (Cat. ab62734, Abcam, Cambridge, U.K., http://www.abcam.com), DDK antibody (Cat. TA50011, OriGene), OCT4 antibody (Cat. 4286, Cell Signaling), SOX2 antibody (Cat. 2748, Cell Signaling), normal mouse IgG (Santa Cruz Biotechnology) or normal rabbit IgG (Santa Cruz Biotechnology) were mixed and incubated to form IP antibodyIP matrix complex and the control-IP matrix complex, respectively.

Techniques: Expressing

Journal: Stem cells (Dayton, Ohio)

Article Title: DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.

doi: 10.1002/stem.2252

Figure Lengend Snippet: Figure 4. Regulation of NANOG protein levels by DPPA5 in human pluripotent stem cells (hPSCs). (A): Relative transcript levels of DPPA5, NANOG, OCT4, SOX2, KLF4, and C-MYC between control fibroblasts and with DPPA5 overexpression. (B): Western blot analysis and relative signal intensity of DPPA5, NANOG, OCT4, SOX2, KLF4, and C-MYC proteins between control fibroblasts and fibroblasts with DPPA5 overexpression. b-Actin was used as a loading control. (C): Relative transcript levels of DPPA5, NANOG, OCT4, SOX2, KLF4, and C- MYC between control hESCs and hESCs with DPPA5 overexpression. (D): Western blot analysis and relative signal intensity of DPPA5, NANOG, OCT4, SOX2, KLF4, and C-MYC proteins between control hESCs and hESCs with DPPA5 overexpression. b-Actin was used as a loading control. Plot data are presented as mean 6 SD from three independent experiments (*, p < .05). Abbreviations: DPPA5, develop- mental pluripotency associated 5; hESCs, human embryonic stem cells.

Article Snippet: Meanwhile, NANOG antibody (Cat. ab62734, Abcam, Cambridge, U.K., http://www.abcam.com), DDK antibody (Cat. TA50011, OriGene), OCT4 antibody (Cat. 4286, Cell Signaling), SOX2 antibody (Cat. 2748, Cell Signaling), normal mouse IgG (Santa Cruz Biotechnology) or normal rabbit IgG (Santa Cruz Biotechnology) were mixed and incubated to form IP antibodyIP matrix complex and the control-IP matrix complex, respectively.

Techniques: Control, Over Expression, Western Blot

Journal: Stem cells (Dayton, Ohio)

Article Title: DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.

doi: 10.1002/stem.2252

Figure Lengend Snippet: Figure 6. DPPA5 enhances the function of NANOG in human pluripotent stem cells (hPSCs). (A): The expression of NANOG target genes regulated by DPPA5. Relative transcript level of SALL4 was higher, while GATA6 and SOCS3 were lower in hESCs with DPPA5 over- expression compared to the control hESCs. (B): hESC differentiation regulated by DPPA5. After induced neuroectoderm differentiation in hESCs, the expression levels of pluripotency markers such as OCT4 and SOX2 decreased, while neuroectordern markers SOX1 and NEU- ROD1 increased in both control and DPPA5 overexpressed cells. In neuroectoderm differentiated cells, the expression levels of SOX1 and NEUROD1 were lower in DPPA5 overexpressed cells compared to controls. Data presented as mean 6 SD from three independent experiments (*, p < .05). Abbreviations: bFGF, basic fibroblast growth factor; DPPA5, developmental pluripotency associated 5; hESCs, human embryonic stem cells.

Article Snippet: Meanwhile, NANOG antibody (Cat. ab62734, Abcam, Cambridge, U.K., http://www.abcam.com), DDK antibody (Cat. TA50011, OriGene), OCT4 antibody (Cat. 4286, Cell Signaling), SOX2 antibody (Cat. 2748, Cell Signaling), normal mouse IgG (Santa Cruz Biotechnology) or normal rabbit IgG (Santa Cruz Biotechnology) were mixed and incubated to form IP antibodyIP matrix complex and the control-IP matrix complex, respectively.

Techniques: Expressing, Over Expression, Control

Journal: Stem cells (Dayton, Ohio)

Article Title: DPPA5 Supports Pluripotency and Reprogramming by Regulating NANOG Turnover.

doi: 10.1002/stem.2252

Figure Lengend Snippet: Figure 7. DPPA5 (Developmental Pluripotency Associated 5) overexpression has an additive effect in hiPSC reprogramming. Day 6, day 9, and day 12-DPPA5 added groups (d61D, d91D, d121D) had higher iPSC colony number compared to control group and day 0-DPPA5 added group (d01D). In control group (Con), fibroblasts were infected on day 0 with retroviruses encod- ing KLF4, C-MYC, OCT4, and SOX2 (KMOS) while in experimental groups in addition to this cocktail of reprogramming factors, cells were also infected with retrovirus encoding DPPA5 on day 0 (d01D), day 6 (d61D), day 9 (d91D), and day 12 (d121D). hiPSC colonies were identified by positive alkaline phosphatase staining and morphological characteristics of colonies, quantified and com- pared among groups. Plot data are presented as mean 6 SD from three independent experiments (*, p < .05). Abbreviation: hiPSC, human induced pluripotent stem cell.

Article Snippet: Meanwhile, NANOG antibody (Cat. ab62734, Abcam, Cambridge, U.K., http://www.abcam.com), DDK antibody (Cat. TA50011, OriGene), OCT4 antibody (Cat. 4286, Cell Signaling), SOX2 antibody (Cat. 2748, Cell Signaling), normal mouse IgG (Santa Cruz Biotechnology) or normal rabbit IgG (Santa Cruz Biotechnology) were mixed and incubated to form IP antibodyIP matrix complex and the control-IP matrix complex, respectively.

Techniques: Over Expression, Control, Infection, Staining